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  Indian J Med Microbiol
 

Figure 1: Flow chart of an IHC procedure on the formalin-fixed paraffin-embedded section. Starting from tissue fixation in 10% buffered formalin, immunohistochemistry shares many of the standard steps in classic histotechnology. Antigen retrieval (heat-induced epitope retrieval), choice of the primary antibody, and polymer-based detection system (secondary antibody) are critical for the success of IHC. After antibody incubations, plenty washing steps with phosphate buffered saline should follow but use Tris-buffered saline instead when working with alkaline phosphatase-conjugated antibodies. Digital pathology solutions have made image analysis quantitative in IHC. IHC: Immunohistochemistry

Figure 1: Flow chart of an IHC procedure on the formalin-fixed paraffin-embedded section. Starting from tissue fixation in 10% buffered formalin, immunohistochemistry shares many of the standard steps in classic histotechnology. Antigen retrieval (heat-induced epitope retrieval), choice of the primary antibody, and polymer-based detection system (secondary antibody) are critical for the success of IHC. After antibody incubations, plenty washing steps with phosphate buffered saline should follow but use Tris-buffered saline instead when working with alkaline phosphatase-conjugated antibodies. Digital pathology solutions have made image analysis quantitative in IHC. IHC: Immunohistochemistry