• Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
REVIEW
Year : 2017  |  Volume : 3  |  Issue : 5  |  Page : 174-180

Recent progress in technological improvement and biomedical applications of the clustered regularly interspaced short palindromic repeats/cas system


1 Translational Medicine Institute, National and Local Joint Engineering Laboratory of High-Through Molecular Diagnostic Technology, The First People's Hospital of Chenzhou, University of South China, Chenzhou; Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, University of South China, Hengyang, Hunan, China
2 Translational Medicine Institute, National and Local Joint Engineering Laboratory of High-Through Molecular Diagnostic Technology, The First People’s Hospital of Chenzhou, University of South China, Chenzhou, Hunan, China
3 Department of Pharmacology, Southern Illinois University, School of Medicine, Springfield, IL, USA
4 Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, University of South China, Hengyang, Hunan, China
5 Division of Stem Cell Regulation and Application, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China
6 Translational Medicine Institute, National and Local Joint Engineering Laboratory of High-Through Molecular Diagnostic Technology, The First People's Hospital of Chenzhou, University of South China; Center for Pathology, The First People's Hosptial of Chenzhou, Southern Medical University, Chenzhou, Hunan, China

Correspondence Address:
Di-Xian Luo
Center for Pathology, The First People's Hospital of Chenzhou, Southern Medical University, Chenzhou 423000, Hunan
China
Duanfang Liao
Division of Stem Cell Regulation and Application, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, Hunan
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ctm.ctm_22_17

Rights and Permissions

The adaptive immune systems of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (Cas) selectively destroy nonnative DNA and defend almost all archaea and about half of the bacteria against infections. In the past years, the system has been genetically engineered to a powerful genome editing tool for a wide variety of organisms. Recently, many progresses have been made in the CRISPR-Cas systems. These improvements include applications in editing multiple genes, correcting mutation genes with one base difference, targeting nondividing cells, reducing off-target, and editing RNAs. The biomedical applications of the technology are to edit not only cells but also embryos in clinical settings. In this review, we briefly introduce the improvements of CRISPR-Cas9 gene editing methods and summarize the recent advances of this technology.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed229    
    Printed1    
    Emailed0    
    PDF Downloaded47    
    Comments [Add]    

Recommend this journal